3IQ2
Human sorting nexin 7, phox homology (PX) domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.03790 |
| Spacegroup name | P 32 |
| Unit cell lengths | 57.300, 57.300, 97.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.650 - 1.700 |
| R-factor | 0.16638 |
| Rwork | 0.165 |
| R-free | 0.19963 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ocs |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.497 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.087 | 0.561 |
| Number of reflections | 39129 | |
| <I/σ(I)> | 13.1 | 2.8 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 2M AmmoniumSulfate, 0.1M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
