3IP6
Structure of Atu2422-GABA receptor in complex with proline
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 119.230, 43.020, 66.300 |
Unit cell angles | 90.00, 109.66, 90.00 |
Refinement procedure
Resolution | 32.280 - 1.400 |
R-factor | 0.1996 |
Rwork | 0.198 |
R-free | 0.23030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ip5 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.041 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.280 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 61219 | |
<I/σ(I)> | 9.1 | |
Completeness [%] | 97.6 | 95.1 |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris-HCl, 6.5% glycerol, 2.5 M Ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |