3IOZ
SIVmac239 Nef in complex with a TCR zeta polypeptide DP1 (L51-D93)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Detector technology | CCD |
Collection date | 2008-10-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.290 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 51.638, 51.638, 189.449 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.854 - 3.699 |
R-factor | 0.302 |
Rwork | 0.301 |
R-free | 0.32900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.565 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.830 |
High resolution limit [Å] | 3.700 | 7.960 | 3.700 |
Rmerge | 0.051 | 0.030 | 0.393 |
Number of reflections | 3122 | ||
<I/σ(I)> | 12.8 | ||
Completeness [%] | 99.4 | 95.1 | 100 |
Redundancy | 12.6 | 10.8 | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 277 | 15% PEG 3350, 0.15M KF, 0.1M hepes, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |