3IOX
Crystal Structure of A3VP1 of AgI/II of Streptococcus mutans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.725, 153.057, 49.677 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.590 - 1.800 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.22800 |
| Starting model (for MR) | 1jmm |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.069 | 0.298 |
| Number of reflections | 45578 | |
| <I/σ(I)> | 16.5 | 6.3 |
| Completeness [%] | 90.7 | 87.7 |
| Redundancy | 5.1 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 298 | 30% MME PEG 2000, 0.2M Ammonium sulfate, 0.05M Sodium cacodylate, 0.05M fructose, pH 4.6, VAPOR DIFFUSION, temperature 298K |
