3IO6
Huntingtin amino-terminal region with 17 Gln residues - crystal C92-a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 163.401, 101.242, 138.190 |
| Unit cell angles | 90.00, 92.00, 90.00 |
Refinement procedure
| Resolution | 38.670 - 3.700 |
| R-factor | 0.24754 |
| Rwork | 0.245 |
| R-free | 0.29262 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.938 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 3.700 |
| Number of reflections | 24500 |
| <I/σ(I)> | 7 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | pH 7.2, VAPOR DIFFUSION, HANGING DROP |
