3IO5
Crystal Structure of a dimeric form of the uvsX Recombinase core domain from Enterobacteria Phage T4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 |
| Unit cell lengths | 95.974, 95.974, 131.274 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.560 - 2.400 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.24800 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.759 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.462 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 28005 | |
| <I/σ(I)> | 29.5 | 3.7 |
| Completeness [%] | 98.1 | 84.7 |
| Redundancy | 7.9 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | Ammonium sulfate, Ammonium phosphate, DTT, HEPES-Na, pH 8.0, vapor diffusion, hanging drop, temperature 291K |
