3IO4
Huntingtin amino-terminal region with 17 Gln residues - Crystal C90
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.55 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 162.950, 101.210, 142.050 |
Unit cell angles | 90.00, 90.42, 90.00 |
Refinement procedure
Resolution | 37.420 - 3.630 |
R-factor | 0.25397 |
Rwork | 0.251 |
R-free | 0.30354 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ior |
RMSD bond length | 0.009 |
RMSD bond angle | 1.180 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 3.000 |
Number of reflections | 23000 |
<I/σ(I)> | 5.5 |
Completeness [%] | 90.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 12% polyethylene glycol, 200 mM Zn Acetate, 200 mM Sodium Acetate, 100 mM Sodium Cacodylate pH 6.5 to 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |