3IO1
Crystal Structure of Aminobenzoyl-glutamate utilization protein from Klebsiella pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 183.390, 48.519, 107.998 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.020 - 2.500 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.26000 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.091 | 0.410 |
| Number of reflections | 34494 | |
| <I/σ(I)> | 13.3 | 1 |
| Completeness [%] | 99.4 | 97.7 |
| Redundancy | 10.5 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 0.2 M Malic acid, 20% PEG 3350, 0.01 M Yttrium Chloride, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
