3INZ
H57T Hfq from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.290, 71.160, 104.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.1527 |
| Rwork | 0.149 |
| R-free | 0.21790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u1s |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.315 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.4_4)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.362 |
| Number of reflections | 50597 | |
| <I/σ(I)> | 27.9 | 3.9 |
| Completeness [%] | 94.1 | 80.4 |
| Redundancy | 6.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 295 | 4 mg/ml protein, 50 mM NaCl, 100 mM NH4Cl, 7,5% MMEPEG 2000, 50 mM Tris HCl, 10 mM CdCl2, pH 8.5, VAPOR DIFFUSION, temperature 295K |
