3IMP
New crystal form of the C-terminal domain of Helicobacter pylori MotB (residues 125-256)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-13 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 107.560, 100.336, 108.490 |
Unit cell angles | 90.00, 119.51, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.500 |
R-factor | 0.189 |
Rwork | 0.186 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cyp |
RMSD bond length | 0.014 |
RMSD bond angle | 1.429 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.105 | 0.363 |
Number of reflections | 69194 | |
<I/σ(I)> | 10.4 | 2.8 |
Completeness [%] | 99.0 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 15% PEG3350, 200mM sodium tartrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |