3IMM

Crystal structure of Putative glycosyl hydrolase (YP_001301887.1) from Parabacteroides distasonis ATCC 8503 at 2.00 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-15
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97805,0.97874
Spacegroup name	P 31
Unit cell lengths	45.404, 45.404, 241.069
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.105 - 2.000
R-factor	0.166
Rwork	0.163
R-free	0.22200
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.529
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.5)
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.105	28.100	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.078	0.057	0.368
Total number of observations		1165	6906
Number of reflections	37531
<I/σ(I)>	9.7	22.1	2.2
Completeness [%]	99.7	96.5	96.9
Redundancy	2.9	2.8	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.2000M NaCl, 30.0000% PEG-3000, 0.1M TRIS pH 7.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K