3IM6
Crystal structure of mouse Ryanodine Receptor 2 mutant V186M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97949 |
| Spacegroup name | I 41 |
| Unit cell lengths | 112.512, 112.512, 36.751 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.560 - 1.700 |
| R-factor | 0.20336 |
| Rwork | 0.201 |
| R-free | 0.24403 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xzz IP3 receptor NTD |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.439 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.560 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 25132 | |
| <I/σ(I)> | 20.6 | 2.4 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.3 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 294 | 0.2M sodium malonate, 5-15% saturated ammonium sulphate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
