3IM5
Crystal structure of mouse Ryanodine Receptor 2 (residues 1-217)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.971, 39.030, 107.623 |
| Unit cell angles | 90.00, 131.81, 90.00 |
Refinement procedure
| Resolution | 80.320 - 2.550 |
| R-factor | 0.22458 |
| Rwork | 0.221 |
| R-free | 0.28777 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RyR2 NTD V186M mutant |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.231 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.320 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 15222 | |
| <I/σ(I)> | 19.2 | 2.2 |
| Completeness [%] | 93.6 | 58.5 |
| Redundancy | 3.4 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 294 | 0.5-1.5M sodium malonate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
