3ILN
X-ray structure of the laminarinase from Rhodothermus marinus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.427 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.218, 108.288, 64.588 |
Unit cell angles | 90.00, 113.90, 90.00 |
Refinement procedure
Resolution | 25.922 - 1.950 |
R-factor | 0.1586 |
Rwork | 0.157 |
R-free | 0.18960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hyk |
RMSD bond length | 0.006 |
RMSD bond angle | 1.024 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.200 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.104 | 0.372 |
Number of reflections | 46598 | |
<I/σ(I)> | 5.9 | 1.9 |
Completeness [%] | 97.8 | 94.9 |
Redundancy | 4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 6% PEG 3350, 120mM phosphate buffer, 50mM potassium phosphate, 2% ethanol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |