3ILH
Crystal structure of Two component response regulator from Cytophaga hutchinsonii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-31 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 63.671, 63.671, 158.388 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.590 |
| R-factor | 0.235 |
| Rwork | 0.232 |
| R-free | 0.28800 |
| Structure solution method | SAD |
| RMSD bond length | 0.039 |
| RMSD bond angle | 3.291 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.680 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.072 | 0.810 |
| Number of reflections | 6499 | |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 55.9 | 47.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 298 | 0.1M Sodium Acetate trihydrate, pH4.5, 3.0M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
