3ILD

Structure of ORF157-K57A from Acidianus filamentous virus 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-14
Detector	ADSC QUANTUM 4
Wavelength(s)	0.93
Spacegroup name	P 31 2 1
Unit cell lengths	64.757, 64.757, 85.743
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.000 - 3.100
R-factor	0.27335
Rwork	0.271
R-free	0.30932
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ii2
RMSD bond length	0.012
RMSD bond angle	1.331
Data reduction software	XDS
Data scaling software	SCALA
Refinement software	REFMAC (5.5.0088)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	3.270
High resolution limit [Å]	3.100	3.100
Rmerge	0.072	0.404
Number of reflections	4047
<I/σ(I)>	29.2	6.8
Completeness [%]	100.0	100
Redundancy	11.6	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	20% PEG 8000, 0.2M MgCl2, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K