3IKN
Crystal structure of galactose bound trimeric human lung surfactant protein D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-05 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.740, 108.580, 55.880 |
| Unit cell angles | 90.00, 91.41, 90.00 |
Refinement procedure
| Resolution | 55.860 - 1.600 |
| Rwork | 0.201 |
| R-free | 0.21500 |
| Structure solution method | isomorphous to native crystals |
| Starting model (for MR) | 1pw9 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (CCP4_3.1.4) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.900 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.040 | 0.119 |
| Number of reflections | 83548 | |
| <I/σ(I)> | 18.5 | 8.5 |
| Completeness [%] | 95.8 | 84.6 |
| Redundancy | 4.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 4000, tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
