3IKB
The structure of a conserved protein from Streptococcus mutans UA159.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 66.372, 66.372, 197.818 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.930 - 1.670 |
| R-factor | 0.18613 |
| Rwork | 0.185 |
| R-free | 0.21421 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.504 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 1.700 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.107 | 0.873 |
| Number of reflections | 51476 | |
| <I/σ(I)> | 32.7 | 2.5 |
| Completeness [%] | 97.6 | 97.5 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 297 | 0.1M Tris-sodium citrate, 20%w/v PEG6000, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
