3IJU
Chicken egg white lysozyme by highly ordered APA (Anodic Porous Alumina) nanotemplate crystallization method
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID13 |
Synchrotron site | ESRF |
Beamline | ID13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-31 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9465 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.064, 79.064, 37.544 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.570 - 1.600 |
R-factor | 0.226 |
Rwork | 0.225 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yvb |
RMSD bond length | 0.010 |
RMSD bond angle | 1.252 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.907 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.099 | 0.221 |
Total number of observations | 176731 | 26673 |
Number of reflections | 16036 | |
<I/σ(I)> | 21.6 | 2.8 |
Completeness [%] | 98.9 | 100 |
Redundancy | 11 | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | 0.04N NaOAc/HOAc buffer, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |