3IJO
Crystal structure of the AMPA subunit GluR2 bound to the allosteric modulator, althiazide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-23 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.977 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 47.550, 114.558, 164.597 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.078 - 2.003 |
R-factor | 0.197 |
Rwork | 0.196 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dp6 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.090 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.159 | 0.698 |
Number of reflections | 61344 | |
<I/σ(I)> | 6.1 | 2.36 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 6.6 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 16-18 PEG8K, 0.1 M Na Cacodylate, 0.1-0.15 zinc acetate, pH 6.5, vapor diffusion, hanging drop, temperature 277K |