3IJM
The structure of a restriction endonuclease-like fold superfamily protein from Spirosoma linguale.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-31 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97950, 0.97992 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.770, 65.849, 68.917 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.420 - 1.700 |
R-factor | 0.168 |
Rwork | 0.166 |
R-free | 0.19900 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.382 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.058 | 0.477 |
Number of reflections | 33607 | |
<I/σ(I)> | 10.1 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.2 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 279 | 0.1M NaCl, 0.1M Bis Tris pH6.5, 1.5M (NH4)2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 279K |