3IJF
Crystal structure of cytidine deaminase from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-02-07 |
Detector | MAC Science DIP-3000 |
Wavelength(s) | 1.437 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 63.705, 75.338, 55.116 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.240 - 1.990 |
R-factor | 0.19264 |
Rwork | 0.190 |
R-free | 0.24015 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fr5 |
RMSD bond length | 0.019 |
RMSD bond angle | 2.161 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 22.270 |
High resolution limit [Å] | 1.990 |
Rmerge | 0.086 |
Number of reflections | 9374 |
<I/σ(I)> | 10 |
Completeness [%] | 97.0 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM HEPES and 4.3 M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |