3IIY
Crystal structure of Eed in complex with a trimethylated histone H1K26 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.573, 85.180, 91.352 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.650 |
| R-factor | 0.18418 |
| Rwork | 0.180 |
| R-free | 0.25924 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SeMet Eed Structure |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.215 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.119 | 0.389 |
| Number of reflections | 12915 | |
| <I/σ(I)> | 11.3 | 3.2 |
| Completeness [%] | 98.1 | 98.8 |
| Redundancy | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 4M Sodium formate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
