3IIW
Crystal structure of Eed in complex with a trimethylated histone H3K27 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9700 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.745, 85.118, 91.246 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.15896 |
| Rwork | 0.157 |
| R-free | 0.19315 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SeMet Eed structure |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.486 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.880 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.073 | 0.279 |
| Number of reflections | 40269 | |
| <I/σ(I)> | 11.3 | 2.8 |
| Completeness [%] | 97.8 | 85.8 |
| Redundancy | 3.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 4.0 M Sodium formate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
