3IIS
Structure of the reconstituted Peridinin-Chlorophyll a-Protein (RFPCP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9797 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.690, 81.623, 75.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.850 - 1.400 |
| R-factor | 0.15353 |
| Rwork | 0.152 |
| R-free | 0.18725 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ppr N-Domain of M monomer |
| RMSD bond length | 0.017 |
| RMSD bond angle | 3.339 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.900 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 40040 | |
| <I/σ(I)> | 14.07 | 4.04 |
| Completeness [%] | 95.7 | 92 |
| Redundancy | 7.6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 296 | 0.1M CdCl2, 0,1M Sodium Acetate pH 4.6, 20-24% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
