3II3
Structure of ORF157 from Acidianus filamentous Virus 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 65.445, 65.445, 85.604 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.700 |
| R-factor | 0.27057 |
| Rwork | 0.270 |
| R-free | 0.28144 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ii2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.558 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.084 | 0.460 |
| Number of reflections | 6151 | |
| <I/σ(I)> | 10.3 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.2 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.9M LiSO4, 0.01M NiCl2, 0.1M Tris, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
