3IHZ
Crystal structure of the FK506 binding domain of Plasmodium vivax FKBP35 in complex with FK506
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-06-08 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.900, 40.900, 56.900 |
| Unit cell angles | 90.00, 109.50, 90.00 |
Refinement procedure
| Resolution | 21.220 - 1.670 |
| R-factor | 0.19009 |
| Rwork | 0.188 |
| R-free | 0.23277 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vn1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.502 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.220 | 1.750 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.029 | 0.150 |
| Number of reflections | 28288 | |
| <I/σ(I)> | 30.4 | 8.2 |
| Redundancy | 4.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 277 | 100mM BICINE pH 9.0, 2.4M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |
