3IHO
The C-terminal glycosylase domain of human MBD4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Spacegroup name | H 3 |
| Unit cell lengths | 83.541, 83.541, 73.956 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.710 - 2.700 |
| R-factor | 0.18735 |
| Rwork | 0.182 |
| R-free | 0.29003 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.978 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 51.710 | 40.000 | 2.800 |
| High resolution limit [Å] | 2.697 | 5.810 | 2.697 |
| Rmerge | 0.090 | 0.039 | 0.315 |
| Number of reflections | 5287 | ||
| <I/σ(I)> | 6.3 | ||
| Completeness [%] | 93.7 | 99.9 | 64.3 |
| Redundancy | 3.7 | 4.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 25% PEG3350, 0.2M Li Sulfate, 0.1M Hepes pH 7.5, VAPOR DIFFUSION, SITTING DROP |
