3IHG
Crystal structure of a ternary complex of aklavinone-11 hydroxylase with FAD and aklavinone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-15 |
| Wavelength(s) | 1.0159 |
| Spacegroup name | P 63 |
| Unit cell lengths | 183.035, 183.035, 99.639 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.680 - 2.490 |
| R-factor | 0.20663 |
| Rwork | 0.204 |
| R-free | 0.25520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.877 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.620 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Number of reflections | 65078 | |
| <I/σ(I)> | 16 | 4.7 |
| Completeness [%] | 97.1 | 81 |
| Redundancy | 5.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 20mM Tris, 2.1M ammonium sulfate, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
