3IGX
1.85 Angstrom Resolution Crystal Structure of Transaldolase B (talA) from Francisella tularensis.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-24 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97921 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.492, 75.859, 164.771 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.410 - 1.850 |
R-factor | 0.15145 |
Rwork | 0.150 |
R-free | 0.18598 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.290 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CRANK |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.075 | 0.585 |
Number of reflections | 62081 | |
<I/σ(I)> | 28.6 | 3 |
Completeness [%] | 99.4 | 93 |
Redundancy | 9.6 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | Protein solution: 0.3M NaCl, 10mM HEPES (pH 7.5). Screen solution: 50mM Potassium Dhydrogen phosphate, 20% PEG 8000; Cryo solution: 5% Glycerol, 5% Sucrose, 5% Ethelene Glycol in screen solution, VAPOR DIFFUSION, HANGING DROP, temperature 295K |