3IGX

1.85 Angstrom Resolution Crystal Structure of Transaldolase B (talA) from Francisella tularensis.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-24
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97921
Spacegroup name	P 21 21 21
Unit cell lengths	56.492, 75.859, 164.771
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.410 - 1.850
R-factor	0.15145
Rwork	0.150
R-free	0.18598
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.290
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CRANK
Refinement software	REFMAC (5.5.0088)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.880
High resolution limit [Å]	1.850	1.850
Rmerge	0.075	0.585
Number of reflections	62081
<I/σ(I)>	28.6	3
Completeness [%]	99.4	93
Redundancy	9.6	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	Protein solution: 0.3M NaCl, 10mM HEPES (pH 7.5). Screen solution: 50mM Potassium Dhydrogen phosphate, 20% PEG 8000; Cryo solution: 5% Glycerol, 5% Sucrose, 5% Ethelene Glycol in screen solution, VAPOR DIFFUSION, HANGING DROP, temperature 295K