3IGS
Structure of the Salmonella enterica N-acetylmannosamine-6-phosphate 2-epimerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-06-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.290, 63.320, 41.800 |
| Unit cell angles | 90.00, 91.28, 90.00 |
Refinement procedure
| Resolution | 37.700 - 1.500 |
| R-factor | 0.148 |
| Rwork | 0.147 |
| R-free | 0.17700 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.371 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.095 | 0.393 |
| Number of reflections | 74625 | |
| <I/σ(I)> | 26.7 | 3.33 |
| Completeness [%] | 96.9 | 74.8 |
| Redundancy | 7.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 2M ammonium sulphate, 5% isopropanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
