3IGM
A 2.2A crystal structure of the AP2 domain of PF14_0633 from P. falciparum, bound as a domain-swapped dimer to its cognate DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97886 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 43.810, 58.821, 177.296 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.550 - 2.200 |
| R-factor | 0.21681 |
| Rwork | 0.214 |
| R-free | 0.26268 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.348 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 10478 | |
| <I/σ(I)> | 32.1 | 4.5 |
| Completeness [%] | 91.9 | 72.4 |
| Redundancy | 11 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 295 | 0.5M-1.25M 1,6-hexanediol 100mM NaOAc pH 5.1 @295K 10mM CoCl2 10-20% ethylene glycol, VAPOR DIFFUSION, HANGING DROP |
