3IGM
A 2.2A crystal structure of the AP2 domain of PF14_0633 from P. falciparum, bound as a domain-swapped dimer to its cognate DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97886 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 43.810, 58.821, 177.296 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.550 - 2.200 |
R-factor | 0.21681 |
Rwork | 0.214 |
R-free | 0.26268 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.348 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 10478 | |
<I/σ(I)> | 32.1 | 4.5 |
Completeness [%] | 91.9 | 72.4 |
Redundancy | 11 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 295 | 0.5M-1.25M 1,6-hexanediol 100mM NaOAc pH 5.1 @295K 10mM CoCl2 10-20% ethylene glycol, VAPOR DIFFUSION, HANGING DROP |