3IGI
Tertiary Architecture of the Oceanobacillus Iheyensis Group II Intron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.092, 94.969, 225.972 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.705 - 3.125 |
| R-factor | 0.1844 |
| Rwork | 0.183 |
| R-free | 0.21830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eoh |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.259 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | PHENIX ((phenix.refine: 1.4_70)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.705 | 3.217 |
| High resolution limit [Å] | 3.125 | 3.125 |
| Number of reflections | 34359 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | group II intron | 10 mg/ml | |
| 2 | 1 | 1 | magnesium chloride | 40 mM | |
| 3 | 1 | 1 | sodium cacodylate pH 6.5 | 5 mM | |
| 4 | 1 | 2 | PEG 3350 | 6% | |
| 5 | 1 | 2 | magnesium acetate | 150mM | |
| 6 | 1 | 2 | KCl | 350 mM | |
| 7 | 1 | 2 | Na-HEPES pH 7.0 | 50 mM | |
| 8 | 1 | 3 | spermine | 0.5 mM |
