3IGF
Crystal Structure of the All4481 protein from Nostoc sp. PCC 7120, Northeast Structural Genomics Consortium Target NsR300
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97908 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 124.170, 55.553, 122.363 |
Unit cell angles | 90.00, 98.87, 90.00 |
Refinement procedure
Resolution | 19.960 - 2.000 |
R-factor | 0.19 |
Rwork | 0.189 |
R-free | 0.23300 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.700 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SnB (then SOLVE/RESOLVE) |
Refinement software | CNS (1.2 & XtalView) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.063 | 0.288 |
Number of reflections | 110202 | |
<I/σ(I)> | 18.8 | 3.9 |
Completeness [%] | 99.4 | 99.1 |
Redundancy | 3.3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.15 | 291 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: 100mM MES (pH 6.15), 18% PEG3350, and 100mM potassium nitrate., VAPOR DIFFUSION, HANGING DROP, temperature 291K |