3IGE

Small outer capsid protein (Soc) from bacteriophage RB69

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-04-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	39.220, 88.613, 81.944
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.595 - 2.254
R-factor	0.2189
Rwork	0.217
R-free	0.26270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	structure of the Soc protein from bacteriophage RB69 (hexagonal crystal form).
RMSD bond length	0.002
RMSD bond angle	0.421
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	23.600
High resolution limit [Å]	2.540
Number of reflections	7033
<I/σ(I)>	21.2
Completeness [%]	99.6
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	30% PEG 8000; 0.1 M imidazole; 0.2 M NaCl., VAPOR DIFFUSION, HANGING DROP, temperature 277K