3IG3

Crystal structure of mouse Plexin A3 intracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2008-01-01
Detector	CUSTOM-MADE
Wavelength(s)	0.97926
Spacegroup name	C 1 2 1
Unit cell lengths	147.789, 47.203, 101.178
Unit cell angles	90.00, 118.69, 90.00

Refinement procedure

Resolution	34.082 - 1.990
R-factor	0.19
Rwork	0.188
R-free	0.22300
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.719
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	1.990	4.310	1.990
Rmerge	0.050	0.032	0.355
Number of reflections	41961
<I/σ(I)>	15.7
Completeness [%]	99.6	99	97.4
Redundancy	4.7	4.5	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.75	293	100 mM Bis-Tris Propane, 200 mM NaNO3 and 20% PEG3350, pH 7.75, vapor diffusion, hanging drop, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.75	293	100 mM Bis-Tris Propane, 200 mM NaNO3 and 20% PEG3350, pH 7.75, vapor diffusion, hanging drop, temperature 293K