3IFW
Crystal structure of the S18Y variant of ubiquitin carboxy terminal hydrolase L1 bound to ubiquitin vinylmethylester.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-27 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0032 |
Spacegroup name | H 3 2 |
Unit cell lengths | 87.304, 87.304, 193.544 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.21105 |
Rwork | 0.209 |
R-free | 0.25627 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ETL PDB ENTRY 1XD3 CHAIN B |
RMSD bond length | 0.007 |
RMSD bond angle | 0.896 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.094 | 0.600 |
Number of reflections | 11248 | |
<I/σ(I)> | 21 | 3 |
Completeness [%] | 98.5 | 99.8 |
Redundancy | 9.9 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 2.4 M ammonium sulfate, 0.1M BICINE, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |