3IFT
Crystal structure of glycine cleavage system protein H from Mycobacterium tuberculosis, using X-rays from the Compact Light Source.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.81836 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.450, 51.010, 32.570 |
| Unit cell angles | 90.00, 95.10, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.185 |
| Rwork | 0.181 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hgb |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.586 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.095 | 0.385 |
| Number of reflections | 9535 | |
| <I/σ(I)> | 10.17 | 3.2 |
| Completeness [%] | 98.8 | 99.3 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 290 | 1.5M SODIUM CITRATE, 50MM BISTRIS PH 6.3; MYTUD.01046.A AT 27MG/ML, , VAPOR DIFFUSION, SITTING DROP, temperature 290K |
