3IFQ
Interction of plakoglobin and beta-catenin with desmosomal cadherins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2008-12-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.043, 76.150, 122.940 |
| Unit cell angles | 90.00, 97.15, 90.00 |
Refinement procedure
| Resolution | 44.786 - 2.800 |
| R-factor | 0.2005 |
| Rwork | 0.194 |
| R-free | 0.26090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i7w |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.619 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.097 | 0.400 |
| Number of reflections | 34257 | |
| <I/σ(I)> | 13.89 | 2.3 |
| Completeness [%] | 99.1 | 99.2 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289.5 | 17% PEG 3350, 0.1M Ammonium sulfate, 0.1M Tris-Cl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289.5K |
