3IF8
Crystal Structure of ZWILCH, a member of the RZZ kinetochore complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 86.600, 86.600, 166.890 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.680 - 2.550 |
| R-factor | 0.231 |
| Rwork | 0.229 |
| R-free | 0.27400 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.358 |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.680 | 2.620 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.042 | 0.325 |
| Number of reflections | 24132 | 3268 |
| <I/σ(I)> | 12.95 | 2.3 |
| Completeness [%] | 99.4 | 99.3 |
| Redundancy | 3.59 | 3.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 293 | 10-20% (w/v) PEG3350, 100mM BisTris Propane, 200mM Na thiocyanate, pH6.25, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
