3IF4
Structure from the mobile metagenome of North West Arm Sewage Outfall: Integron Cassette Protein Hfx_Cass5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9798 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.324, 44.481, 82.462 |
Unit cell angles | 90.00, 109.81, 90.00 |
Refinement procedure
Resolution | 40.226 - 2.181 |
R-factor | 0.1828 |
Rwork | 0.178 |
R-free | 0.23280 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.876 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.180 | 2.200 |
Rmerge | 0.093 | 0.531 |
Number of reflections | 21618 | |
<I/σ(I)> | 34.6 | 2.62 |
Completeness [%] | 97.6 | 68.6 |
Redundancy | 7 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 29% PEG3350, 0.1 M Hepes pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |