3IEV
Crystal Structure of ERA in Complex with MgGNP and the 3' End of 16S rRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.600, 71.600, 43.600 |
| Unit cell angles | 90.00, 101.50, 90.00 |
Refinement procedure
| Resolution | 23.720 - 1.900 |
| R-factor | 0.18546 |
| Rwork | 0.183 |
| R-free | 0.22747 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wf3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.111 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0057) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.093 | 0.501 |
| Number of reflections | 27880 | |
| <I/σ(I)> | 15.3 | 1.9 |
| Completeness [%] | 98.7 | 91 |
| Redundancy | 4.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 0.2 M (NH4)2SO4, 0.1 M Cacodylate and 30% PEG 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
