3IE7
The crystal structure of phosphofructokinase (lin2199) from Listeria innocua in complex with ATP at 1.6A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-16 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.108, 46.244, 57.762 |
| Unit cell angles | 90.00, 102.08, 90.00 |
Refinement procedure
| Resolution | 35.850 - 1.600 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hic |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 48696 | |
| <I/σ(I)> | 15.3 | 8.7 |
| Completeness [%] | 97.3 | 78.5 |
| Redundancy | 6.9 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 292 | 0.2M MgCl2,10mMATP,0.1M Hepes 7.5, 20% PEG 3350%, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
