Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2007-07-14 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.282245 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 143.181, 146.116, 121.004 |
Unit cell angles | 90.00, 109.51, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.730 |
Rwork | 0.218 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dkf |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.830 |
High resolution limit [Å] | 2.730 | 5.880 | 2.730 |
Rmerge | 0.044 | 0.030 | 0.308 |
Number of reflections | 61873 | ||
<I/σ(I)> | 15.1 | ||
Completeness [%] | 99.5 | 98.9 | 99.1 |
Redundancy | 3.8 | 3.7 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 50mM sodium citrate, 0.2M ammonium sulfate, 0.6M lithium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |