3IDI
Crystal structure of the HIV-1 Cross Neutralizing Monoclonal Antibody 2F5 Fab' fragment in complex with gp41 Peptide ALDKWNQ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-06-16 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.500, 76.500, 94.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.150 - 2.100 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.800 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 25395 | |
<I/σ(I)> | 25.3 | 3.2 |
Completeness [%] | 92.5 | 84.8 |
Redundancy | 5.1 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1 M sodium citrate, pH 5.6, 16% 2-propanol, 16% PEG 4000, 0.1% Tween-20, VAPOR DIFFUSION, HANGING DROP, temperature 293K |