3IDF

The Crystal Structure of a USP-like protein from Wolinella succinogenes to 2.0A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9794
Spacegroup name	H 3
Unit cell lengths	89.768, 89.768, 127.286
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.170 - 2.000
R-factor	0.184
Rwork	0.182
R-free	0.21700
RMSD bond length	0.011
RMSD bond angle	1.151
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC (refmac_5.5.0054)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.066	0.057	0.475
Number of reflections	25784
<I/σ(I)>	10.8
Completeness [%]	99.6	97.5	100
Redundancy	3.2	3.3	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	2M Ammonium sulfate, 0.1M Bis-tris pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K