3IDF
The Crystal Structure of a USP-like protein from Wolinella succinogenes to 2.0A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | H 3 |
Unit cell lengths | 89.768, 89.768, 127.286 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.170 - 2.000 |
R-factor | 0.184 |
Rwork | 0.182 |
R-free | 0.21700 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.151 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC (refmac_5.5.0054) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.066 | 0.057 | 0.475 |
Number of reflections | 25784 | ||
<I/σ(I)> | 10.8 | ||
Completeness [%] | 99.6 | 97.5 | 100 |
Redundancy | 3.2 | 3.3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 2M Ammonium sulfate, 0.1M Bis-tris pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |