3IDD
Cofactor-Independent Phosphoglycerate Mutase from Thermoplasma acidophilum DSM 1728
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924, 0.97948 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 88.605, 136.744, 63.289 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.360 - 2.800 |
| R-factor | 0.23439 |
| Rwork | 0.230 |
| R-free | 0.31106 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.610 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.360 | 2.620 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.098 | 0.591 |
| Number of reflections | 22991 | |
| <I/σ(I)> | 1.5 | |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 3.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.20 M NaCl, 0.10 M phosphate citrate, pH 4.2, 10% PEG 3000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
