3IDC
Crystal structure of (102-265)RIIb:C holoenzyme of cAMP-dependent protein kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.5200 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.272, 67.434, 47.325 |
| Unit cell angles | 90.00, 99.51, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.31900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1atp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.780 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.069 | 0.250 |
| Number of reflections | 25383 | |
| <I/σ(I)> | 32.5 | 9.7 |
| Completeness [%] | 95.2 | 88.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION UNDER OIL (VDUO) | 7.5 | 298 | 8% PEG 3350, 40 mM Bis-Tris pH 7.5, 0.05 mM Na Acetate, VAPOR DIFFUSION UNDER OIL (VDUO), temperature 298K |
