3ID9
Crystal structure of a MutT/NUDIX family protein from Bacillus thuringiensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 115.494, 115.494, 106.075 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.390 - 2.550 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.26300 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.390 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.108 | 0.587 |
| Number of reflections | 51688 | |
| <I/σ(I)> | 9.8 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.5 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 1.5M Ammonium sulfate, 25% Glycerol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
