3ID7

Crystal structure of renal dipeptidase from Streptomyces coelicolor A3(2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-17
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	P 31 2 1
Unit cell lengths	96.747, 96.747, 104.721
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.990 - 1.300
R-factor	0.184
Rwork	0.184
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	1.300
Rmerge	0.067
Number of reflections	133376
Completeness [%]	95.9
Redundancy	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	22% polyacrilic acid, 0.1M hepes, 20mM zinc chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K